{"id":717,"date":"2020-12-02T17:19:26","date_gmt":"2020-12-02T08:19:26","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=717"},"modified":"2021-06-24T10:35:32","modified_gmt":"2021-06-24T01:35:32","slug":"%e3%83%97%e3%83%ad%e3%83%a1%e3%82%ac%e3%81%ae%e3%83%ab%e3%82%b7%e3%83%95%e3%82%a7%e3%83%a9%e3%83%bc%e3%82%bc%e8%a9%a6%e9%a8%93%e3%81%a7%e4%bd%bf%e7%94%a8%e3%81%95%e3%82%8c%e3%82%8b%e7%b4%b0%e8%83%9e","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/genetic-reporter\/cell-lysate\/cclr-rlb-plb-glb\/%e3%83%97%e3%83%ad%e3%83%a1%e3%82%ac%e3%81%ae%e3%83%ab%e3%82%b7%e3%83%95%e3%82%a7%e3%83%a9%e3%83%bc%e3%82%bc%e8%a9%a6%e9%a8%93%e3%81%a7%e4%bd%bf%e7%94%a8%e3%81%95%e3%82%8c%e3%82%8b%e7%b4%b0%e8%83%9e","title":{"rendered":"\u30d7\u30ed\u30e1\u30ac\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u8a66\u9a13\u3067\u4f7f\u7528\u3055\u308c\u308b\u7d30\u80de\u6eb6\u89e3\u5264\u306e\u7a2e\u985e\u3068\u305d\u308c\u3089\u306e\u9055\u3044\u306f\u4f55\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n

\u30d7\u30ed\u30e1\u30ac\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u8a66\u9a13\u3067\u306f\u3001Cell Culture Lysis Reagent (CCLR)\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE1531\uff09\u3001Reporter Lysis Buffer (RLB)\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE3971\uff09\u3001Passive Lysis Buffer (PLB)\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE1941\uff09\u3001Glo Lysis Buffer(GLB) (\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1a\uff252661)\u306e4\u7a2e\u985e\u306e\u7d30\u80de\u6eb6\u89e3\u6db2\u304c\u5229\u7528\u3067\u304d\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u7d30\u80de\u6eb6\u89e3\u6db2\u306f\u3001\u4f7f\u7528\u6cd5\u3084\u7279\u5fb4\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u305d\u308c\u3089\u3092\u4ee5\u4e0b\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

CCLR<\/strong>\u306f\u3001Luciferase Assay System\u3067\u52b9\u679c\u7684\u306a\u7d30\u80de\u6eb6\u89e3\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u3001\u307e\u305f\u9ad8\u3044\u30b7\u30b0\u30ca\u30eb\u3068\u4f4e\u3044\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u5f97\u3089\u308c\u308b\u3088\u3046\u306b\u8abf\u88fd\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u3084CAT\u306a\u3069\u3001\u307b\u304b\u306e\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306e\u30a2\u30c3\u30bb\u30a4\u306b\u306f\u5bfe\u5fdc\u3057\u3066\u3044\u307e\u305b\u3093\u3002CCLR\u306b\u542b\u307e\u308c\u308b\u3001\u9ad8\u6fc3\u5ea6\u306e\u754c\u9762\u6d3b\u6027\u5264\u3084DTT\u304c\u4e00\u822c\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u3092\u96e3\u3057\u304f\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30a2\u30c3\u30bb\u30a4\u306e\u524d\u306b\u7d30\u80de\u6eb6\u89e3\u6db2\u30922\u500d\u4ee5\u4e0a\u6c34\u3067\u5e0c\u91c8\u3059\u308c\u3070\u3001\u754c\u9762\u6d3b\u6027\u5264\u5bfe\u5fdc\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u3092\u4f7f\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

RLB<\/strong>\u306f\u3001\u540c\u3058\u7d30\u80de\u6eb6\u89e3\u6db2\u3067\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u3001CAT\u3001\u304a\u3088\u3073\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc \u30ec\u30dd\u30fc\u30bf\u30fc \u30a2\u30c3\u30bb\u30a4\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u7279\u5225\u306b\u8abf\u88fd\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u6eb6\u89e3\u6db2\u306f\u3001CCLR\u3068\u6bd4\u3079\u308b\u3068\u7d30\u80de\u306e\u6eb6\u89e3\u3067\u306f\u52b9\u7387\u304c\u843d\u3061\u308b\u306e\u3067\u3001\u7d30\u80de\u3092\u5b8c\u5168\u306b\u58ca\u3059\u305f\u3081\u306b\u6a5f\u68b0\u7684\u306a\u7834\u58ca\u3084\u51cd\u7d50\u878d\u89e3\u3092\u5fc5\u8981\u3068\u3057\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6fc3\u5ea6\u306f\u754c\u9762\u6d3b\u6027\u5264\u5bfe\u5fdc\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306b\u3088\u308a\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

PLB<\/strong>\u306f\u3001Dual-Luciferase\u00ae<\/sup>\u00a0Reporter Assay System \u3092\u4f7f\u3046\u5834\u5408\u306b\u3001\u30db\u30bf\u30eb\u3001\u30a6\u30df\u30b7\u30a4\u30bf\u30b1\u3001NanoLuc\u306e\u5404\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc \u30a2\u30c3\u30bb\u30a4\u3067\u9ad8\u3044\u30b7\u30b0\u30ca\u30eb\u3068\u4f4e\u3044\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u304c\u5f97\u3089\u308c\u308b\u3088\u3046\u3001\u7279\u5225\u306b\u8abf\u88fd\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u7d30\u80de\u6eb6\u89e3\u6db2\u306f\u3001CAT \u3084 \u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc \u30ec\u30dd\u30fc\u30bf\u30fc \u30a2\u30c3\u30bb\u30a4\u306b\u3082\u5bfe\u5fdc\u3057\u3066\u3044\u307e\u3059\u3002PLB\u306f\u3001\u5f37\u529b\u306a\u6eb6\u89e3\u6db2\u306a\u306e\u3067\u3001\u901a\u5e38\u3001\u7d30\u80de\u3092\u5b8c\u5168\u306b\u6eb6\u89e3\u3059\u308b\u305f\u3081\u306b\u307b\u304b\u306e\u64cd\u4f5c\u3092\u52a0\u3048\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u754c\u9762\u6d3b\u6027\u5264\u9069\u5fdc\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308aPLB\u4e2d\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6fc3\u5ea6\u3092\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n

GLB<\/strong>\u306f\u3001\u5f37\u529b\u3067\u7d30\u80de\u306e\u63bb\u53d6\u308a\u3084\u51cd\u7d50\/\u878d\u89e3\u64cd\u4f5c\u304c\u4e0d\u8981\u3067\u3059\u3002\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u6dfb\u52a0\u3059\u308b\u3060\u3051\u3067\u3001\u901a\u5e385\u5206\u4ee5\u5185\u306b\u7d30\u80de\u3092\u6eb6\u89e3\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u7279\u306b\u30b0\u30ed\u30fc\u30bf\u30a4\u30d7\uff08\u9577\u6642\u9593\u767a\u5149\uff09\u306eBright-Glo\u2122\uff08\uff1e30\u5206\u9593\uff09\u3068Steady-Glo\u00ae<\/sup>\uff08\uff1e5\u6642\u9593\uff09\u7528\u306b\u958b\u767a\u3055\u308c\u3066\u304a\u308a\u3001\u767a\u5149\u6642\u9593\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u306a\u3044\u305f\u3081\u3001\u3069\u3061\u3089\u306b\u4f7f\u7528\u3057\u3066\u3082\u534a\u6e1b\u671f\u306f\u5909\u5316\u3057\u307e\u305b\u3093\u3002\u3069\u3061\u3089\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u5b9a\u91cf\u8a66\u85ac\u3082\u30db\u30e2\u30b8\u30cb\u30a2\u30b9\u30a2\u30c3\u30bb\u30a4\u65b9\u5f0f\u3092\u63a1\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u4f7f\u7528\u306b\u3088\u308a\u5f93\u6765\u306e\u30ce\u30f3-\u30db\u30e2\u30b8\u30cb\u30a2\u30b9\u30a2\u30c3\u30bb\u30a4\uff08\u57f9\u990a\u7d30\u80de\u5168\u4f53\u3092\u6eb6\u89e3\u3057\u3066\u3001\u305d\u306e\u4e00\u90e8\u3092\u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3059\u308b\u65b9\u6cd5\uff09\u306b\u3082\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u9175\u7d20\u306e\u5b89\u5b9a\u6027\u306b\u512a\u308c\u3001\u30db\u30bf\u30eb\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u5834\u5408\u3001\u5ba4\u6e29\u3067\u5c11\u306a\u304f\u3068\u308248\u6642\u9593\u306f\u5b89\u5b9a\u3067\u3059\uff081mg\/ml BSA\u3092\u542b\u30801XGlo Lysis Buffer\u30672.2\u00d710-10<\/sup> M\u306b\u5e0c\u91c8\u3057\u305f\u5834\u5408\uff09\u3002<\/p>\n\n\n\n

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\u3000<\/th>\n CCLR<\/th>\n RLB<\/th>\n PLB<\/th>\n GLB<\/th>\n <\/tr>\n \n
CAT\u30a2\u30c3\u30bb\u30a4<\/td>\n \u00d7<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n <\/tr>\n
\u03b2-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u30a2\u30c3\u30bb\u30a4<\/td>\n \u25b3*<\/sup><\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n \u25b3*<\/sup><\/td>\n <\/tr>\n
GUS\u30a2\u30c3\u30bb\u30a4<\/td>\n \u25cb<\/td>\n \uff0d<\/td>\n \uff0d<\/td>\n \uff0d<\/td>\n <\/tr>\n
\u6eb6\u89e3\u529b<\/td>\n \u25ce<\/td>\n \u25cb<\/td>\n \u25ce<\/td>\n \u25ce<\/td>\n <\/tr>\n
\u9175\u7d20\u5b89\u5b9a\u6027<\/td>\n \u25cb<\/td>\n \uff0d<\/td>\n \u25ce<\/td>\n \u25ce<\/td>\n <\/tr>\n
\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf<\/td>\n \u25b3***<\/sup><\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n <\/tr>\n
\u30a4\u30ce\u30e0\u30a2\u30c3\u30bb\u30a4<\/td>\n \u25cb(ELISE,WES)<\/td>\n \u25cb(ELISE,WES)<\/td>\n \u25cb(ELISE,WES)<\/td>\n \u25cb(WES)<\/td>\n <\/tr>\n
\u80fd\u52d5\u7684\u6eb6\u89e3<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n <\/tr>\n
\u53d7\u52d5\u7684\u6eb6\u89e3<\/td>\n \u25cb<\/td>\n \u00d7<\/td>\n \u25cb<\/td>\n \u25cb<\/td>\n <\/tr>\n
\u6ce1\u7acb\u3061\u9632\u6b62<\/td>\n \u00d7<\/td>\n \u00d7<\/td>\n \u25cb<\/td>\n \uff0d<\/td>\n <\/tr>\n
*<\/sup> \u505c\u6b62\u6db2\u3068\u3057\u30661M Tris(pH8.0)\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408<\/td>\n \uff0d\uff1a\u672a\u78ba\u8a8d<\/td>\n <\/tr>\n
**<\/sup> \u754c\u9762\u6d3b\u6027\u5264\u9069\u5fdc\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u8a66\u85ac\u306e\u5834\u5408<\/td>\n WES\uff1a\u30a6\u30a8\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0<\/td>\n <\/tr>\n
***<\/sup> \u6c34\u30672\u500d\u4ee5\u4e0a\u5e0c\u91c8\u3059\u308c\u3070\u6e2c\u5b9a\u53ef\u80fd<\/td>\n \u3000<\/td>\n <\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u30d7\u30ed\u30e1\u30ac\u306e\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u8a66\u9a13\u3067\u306f\u3001Cell Culture Lysis Reagent (CCLR)\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE1531\uff09\u3001Reporter Lysis Buffer (RLB)\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7\uff1aE3971\uff09\u3001Pas … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[185],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/717"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=717"}],"version-history":[{"count":7,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/717\/revisions"}],"predecessor-version":[{"id":3023,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/717\/revisions\/3023"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=717"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=717"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=717"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}