{"id":852,"date":"2020-12-03T10:04:03","date_gmt":"2020-12-03T01:04:03","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=852"},"modified":"2021-02-16T11:54:39","modified_gmt":"2021-02-16T02:54:39","slug":"q706","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/cytotoxity\/endopoint-assay\/cytotox-96\/q706","title":{"rendered":"CytoTox 96\u00ae \u306e\u4f7f\u7528\u65b9\u6cd5\u3092\u6559\u3048\u3066\u304f\u3060\u3055\u3044\u3002"},"content":{"rendered":"\n<p>CytoTox 96<sup>\u00ae<\/sup>\u306f\u3001\u7d30\u80de\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13\u3001\u5316\u5b66\u7269\u8cea\u306a\u3069\u306e\u85ac\u5264\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13\u3001\u7dcf\u7d30\u80de\u6570\u306e\u6e2c\u5b9a\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>\u7d30\u80de\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13<\/strong>\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u7d30\u80de\u306b\u5bfe\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u7d30\u80de\u306e\u50b7\u5bb3\u4f5c\u7528\u3092\u7d30\u80de\u6bd2\u6027\u3068\u3057\u3066\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u307e\u305a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u7d30\u80de\u6570\u306e\u6700\u9069\u5316\u3092\u884c\u3044\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u8a2d\u5b9a\u3057\u3066\u3001\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3044\u307e\u3059\u3002<br>\uff081\uff09 \u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u7d30\u80de\u306e\u81ea\u7136\u653e\u51faLDH\u91cf\u3000\uff082\uff09 \u5b9f\u9a13\u533a\uff08\u30bf\u30fc\u30b2\u30c3\u30c8\u7d30\u80de\u306b\u5bfe\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u7d30\u80de\u306e\u50b7\u5bb3\u4f5c\u7528\uff09\u3000\uff083\uff09 \u30bf\u30fc\u30b2\u30c3\u30c8\u7d30\u80de\u306e\u81ea\u7136\u653e\u51faLDH\u91cf\u3000\uff084\uff09 \u30bf\u30fc\u30b2\u30c3\u30c8\u7d30\u80de\u304b\u3089\u306e\u6700\u5927LDH\u653e\u51fa\u91cf\u3000\uff085\uff09 Lysis Solution\u306e\u6dfb\u52a0\u306b\u3088\u308b\u6db2\u91cf\u88dc\u6b63\u7528\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3000\uff086\uff09 \u57f9\u990a\u6db2\u306e\u307f\u306e\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\uff08\u30d6\u30e9\u30f3\u30af\uff09<\/p>\n\n\n\n<p>\uff081\uff09\u3001\uff082\uff09\u3001\uff083\uff09\u3001\u3067\u5f97\u3089\u308c\u305f\u305d\u308c\u305e\u308c\u306e\u5438\u5149\u5024\u306e\u5e73\u5747\u5024\u304b\u3089 \uff086\uff09 \u306e\u30d6\u30e9\u30f3\u30af\u306e\u5e73\u5747\u5024\u3092\u5dee\u3057\u5f15\u304d\u307e\u3059\u3002\uff084\uff09 \u3067\u5f97\u3089\u308c\u305f\u5438\u5149\u5024\u306e\u5e73\u5747\u5024\u304b\u3089\uff085\uff09 \u306e\u6db2\u91cf\u88dc\u6b63\u7528\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u5438\u5149\u5024\u306e\u5e73\u5747\u5024\u3092\u5dee\u3057\u5f15\u304d\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u8a08\u7b97\u5f0f\u306b\u3057\u305f\u304c\u3063\u3066\u7d30\u80de\u6bd2\u6027\u306e\u30d1\u30fc\u30bb\u30f3\u30c8\u3092\u7b97\u51fa\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u7d30\u80de\u6bd2\u6027 (%) =<\/p>\n\n\n\n<p>\uff08\u5b9f\u9a13\u533a\uff0d\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u81ea\u7136\u653e\u51fa\u91cf\uff0d\u30bf\u30fc\u30b2\u30c3\u30c8\u81ea\u7136\u653e\u51fa\u91cf\uff09\uff0f\uff08\u30bf\u30fc\u30b2\u30c3\u30c8\u6700\u5927\u653e\u51fa\u91cf\uff0d\u30bf\u30fc\u30b2\u30c3\u30c8\u81ea\u7136\u653e\u51fa\u91cf\uff09<\/p>\n\n\n\n<p>\u00d7 100<\/p>\n\n\n\n<p><strong>\u5316\u5b66\u7269\u8cea\u306a\u3069\u306e\u85ac\u5264\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13<\/strong>\u306f\u3001\u85ac\u5264\u306b\u3088\u308b\u50b7\u5bb3\u4f5c\u7528\u3092\u7d30\u80de\u6bd2\u6027\u3068\u3057\u3066\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u4f7f\u7528\u3059\u308b\u7d30\u80de\u6570\u306e\u6700\u9069\u5316\u3092\u884c\u3044\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u8003\u616e\u3057\u3066\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3044\u307e\u3059\u3002<br>\uff081\uff09 \u7d30\u80de\u304b\u3089\u81ea\u7136\u306b\u653e\u51fa\u3055\u308c\u308bLDH\u91cf\u3000\uff082\uff09 \u8a66\u9a13\u3059\u308b\u5316\u5408\u7269\u3084\u305d\u306e\u4e2d\u9593\u4f1d\u9054\u7269\u8cea\u306e\u5f71\u97ff\u3000\uff083\uff09 \u7d30\u80de\u304b\u3089\u306e\u6700\u5927LDH\u653e\u51fa\u91cf<\/p>\n\n\n\n<p>\u57f9\u5730\u306e\u307f\u304b\u3089\u6210\u308b\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306f&#8221;\u30d6\u30e9\u30f3\u30af&#8221;\u3068\u3057\u3066\u4f7f\u7528\u3057\u307e\u3059\u3002\u7d30\u80de\u6bd2\u6027\u8a66\u85ac(X\u8ef8\uff1a\u5bfe\u6570\u76ee\u76db)\u306b\u5bfe\u3059\u308bLDH\u306e\u653e\u51fa\u91cf(Y\u8ef8\uff1a490nm\u306b\u304a\u3051\u308b\u5438\u5149\u5ea6)\u3092\u30d7\u30ed\u30c3\u30c8\u3057\u3001IC<sub>50<\/sub>\u5024(50%\u306e\u963b\u5bb3\u304c\u8d77\u304d\u308b\u6fc3\u5ea6)\u3092\u8a55\u4fa1\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>\u7dcf\u7d30\u80de\u6570\u306e\u6e2c\u5b9a<\/strong>\u306f\u3001\u3042\u3089\u304b\u3058\u3081\u7d30\u80de\u306e\u5e0c\u91c8\u6db2\u5217\u306bLysis Solution (10X)\u3092\u6dfb\u52a0\u3057\u3001CytoTox 96<sup>\u00ae<\/sup>\u306e\u8a66\u85ac\u3092\u6dfb\u52a0\u3057\u3066\u5f97\u3089\u308c\u308b\u5438\u5149\u5024\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u7d30\u80de\u6570\u3068\u5438\u5149\u5024\u306e\u76f8\u95a2\u95a2\u4fc2\u3092\u6c42\u3081\u307e\u3059\u3002\u7d30\u80de\u6570\u3068LDH\u6d3b\u6027\u3068\u3057\u3066\u793a\u3055\u308c\u308b\u5438\u5149\u5024\u306f\u6bd4\u4f8b\u3057\u307e\u3059\u306e\u3067\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u7d30\u80de\u6570\u3092\u898b\u7a4d\u3082\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u8a73\u3057\u3044\u4f7f\u7528\u65b9\u6cd5\u306f<a href=\"https:\/\/www.promega.jp\/Resources\/Protocols\/Technical%20Bulletins\/0\/CytoTox%2096%20Non%20Radioactive%20Cytotoxicity%20Assay%20Protocol\/\" target=\"_blank\" rel=\"noreferrer noopener\">\u88fd\u54c1\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\uff08TB163\uff09<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>CytoTox 96\u00ae\u306f\u3001\u7d30\u80de\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13\u3001\u5316\u5b66\u7269\u8cea\u306a\u3069\u306e\u85ac\u5264\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13\u3001\u7dcf\u7d30\u80de\u6570\u306e\u6e2c\u5b9a\u306b\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u7d30\u80de\u3092\u4ecb\u3057\u305f\u7d30\u80de\u6bd2\u6027\u8a66\u9a13\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u7d30\u80de\u306b\u5bfe\u3059\u308b\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u7d30\u80de\u306e\u50b7\u5bb3\u4f5c\u7528\u3092\u7d30\u80de\u6bd2\u6027 &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[228],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/852"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=852"}],"version-history":[{"count":2,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/852\/revisions"}],"predecessor-version":[{"id":2451,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/852\/revisions\/2451"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=852"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=852"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=852"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}