{"id":896,"date":"2020-12-03T10:10:35","date_gmt":"2020-12-03T01:10:35","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=896"},"modified":"2021-02-16T09:51:16","modified_gmt":"2021-02-16T00:51:16","slug":"q719","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/cytotoxity\/apotosis\/apo-info\/q719","title":{"rendered":"\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306b\u304a\u3044\u3066\u4f55\u304c\u30a4\u30d9\u30f3\u30c8\u306e\u9032\u884c\u3092\u793a\u3057\u307e\u3059\u304b\u3002\u307e\u305f\u305d\u306e\u7814\u7a76\u306e\u305f\u3081\u306b\u3069\u306e\u3088\u3046\u306a\u30d7\u30ed\u30e1\u30ac\u88fd\u54c1\u304c\u5229\u7528\u3067\u304d\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u305f\u7d30\u80de\u6b7b\u306b\u5bfe\u3059\u308b\u8208\u5473\u306e\u9ad8\u307e\u308a\u306f\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u591a\u304f\u306e\u624b\u6cd5\u306e\u767a\u9054\u3092\u3082\u305f\u3089\u3057\u307e\u3057\u305f\u3002\u30d7\u30ed\u30e1\u30ac\u306f\u3001Anti-ACTIVE\u00ae 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Z-VAD-FMK\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G7231\u3001G7232\uff09\u306f\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306b\u304a\u3051\u308b\u30ab\u30b9\u30d1\u30fc\u30bc\u4f9d\u5b58\u6027\u7d4c\u8def\u3068\u30ab\u30b9\u30d1\u30fc\u30bc\u975e\u4f9d\u5b58\u6027\u7d4c\u8def\u3092\u7814\u7a76\u3059\u308b\u305f\u3081\u306b\u3059\u3050\u3054\u5229\u7528\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u30c1\u30c8\u30af\u30ed\u30fc\u30e0C\u5c40\u5728\u306e\u7814\u7a76\u306e\u305f\u3081\u306b\u3001\u30d7\u30ed\u30e1\u30ac\u306f\u30c1\u30c8\u30af\u30ed\u30fc\u30e0C\u306b\u5bfe\u3057\u3066\u306e\u30cd\u30ba\u30df\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53\u3001Anti-Cytochrome C mAb\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G7421\uff09\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u4e00\u822c\u306b\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u7279\u7570\u7684\u30c0\u30a4\uff08\u4f8b\u3048\u3070CMX-rosamine\uff09\u3092\u4f7f\u3063\u305f\u4e8c\u91cd\u67d3\u8272\u6cd5\u304c\u884c\u308f\u308c\u307e\u3059\u3002\u975e\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u7d30\u80de\u3067\u3001\u30c1\u30c8\u30af\u30ed\u30fc\u30e0C\u6a19\u8b58\u306f\u6591\u70b9\u72b6\u306eCMX-Rosamine \u306e\u67d3\u8272\u3092\u53cd\u6620\u3059\u308b\u67d3\u8272\u3068\u306a\u308b\u306f\u305a\u3067\u3059\u3002\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u7d30\u80de\u3067\u306f\u3001\u30c1\u30c8\u30af\u30ed\u30fc\u30e0C\u306f\u653e\u51fa\u3055\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u67d3\u8272\u306e\u3053\u306e\u540c\u5c40\u5728\u6027\u306f\u898b\u3089\u308c\u306a\u304f\u306a\u308a\u307e\u3059\u3002\u30c1\u30c8\u30af\u30ed\u30fc\u30e0C\u306f\u7d30\u80de\u8cea\u4e2d\u306b\u653e\u51fa\u3055\u308c\u308b\u3068\u4e0d\u5b89\u5b9a\u306b\u306a\u308b\u305f\u3081\u3001\u591a\u304f\u306e\u5834\u5408\u307e\u3063\u305f\u304f\u67d3\u8272\u304c\u78ba\u8a8d\u3055\u308c\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u4f7f\u308f\u308c\u305f\u67d3\u8272\u6761\u4ef6\u304c\u3069\u3093\u306a\u5b58\u5728\u3059\u308b\u30c1\u30c8\u30af\u30ed\u30fc\u30e0C\u3067\u3082\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u3092\u793a\u3059\u305f\u3081\u306b\u3001\u975e\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u4f7f\u3046\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002<\/p>\n\n\n\n<p>\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u5f8c\u671f\u306e\u7814\u7a76\u306e\u305f\u3081\u306b\u3001PARP\u306ep85\u30ab\u30b9\u30d1\u30fc\u30bc\u5206\u89e3\u7523\u7269\u3092\u30d7\u30ed\u30e1\u30ac\u306eAnti-PARP p85 Fragment pAb*\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G7341\uff09\u3092\u4f7f\u3063\u3066\u540c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u88fd\u54c1\u306f\u514d\u75ab\u7d30\u80de\u5316\u5b66\u67d3\u8272\u7528\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u3066\u3001PARP\u306e\u5207\u65ad\u578b\u3092\u7279\u7570\u7684\u306b\u8b58\u5225\u3057\u307e\u3059\u3002\u3053\u306e\u6297\u4f53\u306f\u30a4\u30f3\u30bf\u30af\u30c8\u306a\u5b8c\u5168\u9577\u306ePARP\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u8a8d\u8b58\u3057\u307e\u305b\u3093\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u3053\u306e\u6297\u4f53\u3067\u967d\u6027\u306b\u67d3\u8272\u3055\u308c\u305f\u7d30\u80de\u306f\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u8d77\u3053\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u6700\u5f8c\u306b\u3001\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306e\u9855\u8457\u306a\u7279\u5fb4\u3068\u3055\u308c\u308bDNA\u306e\u65ad\u7247\u5316\u3092\u8abf\u3079\u308b2\u3064\u306eTUNEL\u6cd5\u30ad\u30c3\u30c8\u3082\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002DeadEnd\u2122 Fluorometric TUNEL System\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G3250\uff09\u306f\u3001\u65ad\u7247\u5316DNA\u306e3&#8242;-OH\u672b\u7aef\u306b\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3-12-dUTP*\u3092\u52a0\u3048\u308b\u305f\u3081\u306b\u30bf\u30fc\u30df\u30ca\u30eb\u30c7\u30aa\u30ad\u30b7\u30cc\u30af\u30ec\u30aa\u30c1\u30b8\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\uff08Tdt\uff09\u3092\u4f7f\u3044\u307e\u3059\u3002\u3053\u306e\u6a19\u8b58\u306f\u86cd\u5149\u9855\u5fae\u93e1\u3042\u308b\u3044\u306fFluorescent Activiated Cell Sorting\uff08FACS\u2122\uff09\u5206\u6790\u3092\u4f7f\u3063\u3066\u8996\u899a\u5316\u3055\u308c\u307e\u3059\u3002\u6bd4\u8272\u5b9a\u91cf\u6cd5\u306e\u30a2\u30c3\u30bb\u30a4\u306e\u65b9\u3092\u5e0c\u671b\u3059\u308b\u7814\u7a76\u8005\u306e\u305f\u3081\u306b\u3001DeadEnd\u2122 Colorimetric Apoptosis Detection System\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7 G7130\u3001G7360\uff09\u3067\u306f\u3001\u65ad\u7247\u5316\u3055\u308c\u305fDNA\u306e3&#8242;-OH\u672b\u7aef\u3092\u30d3\u30aa\u30c1\u30f3\u5316\u3055\u308c\u305f\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3067\u30e9\u30d9\u30eb\u3057\u307e\u3059\u3002\u30d3\u30aa\u30c1\u30f3\u5316\u3055\u308c\u305f\u65ad\u7247\u306f\u305d\u306e\u5f8c\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3- HRP\u7d50\u5408\u4f53\u3068\u6c88\u6bbf\u6027\u306eHRP\u57fa\u8cea\uff08DAB\u3068\u904e\u9178\u5316\u6c34\u7d20\uff09\u306b\u3088\u308a\u691c\u51fa\u3067\u304d\u307e\u3059\u3002\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u8d77\u3053\u3057\u3066\u3044\u308b\u6838\u306f\u6a19\u6e96\u7684\u306a\u5149\u5b66\u9855\u5fae\u93e1\u4e0b\u3067\u8996\u899a\u5316\u3055\u308c\u3001\u8336\u8272\u306b\u898b\u3048\u307e\u3059\u3002<\/p>\n\n\n\n<p>CellTiter 96R AQueous One Solution Cell Proliferation Assay\uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G3580\u3001\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G3581\u3001\u30ab\u30bf\u30ed\u30b0\u756a\u53f7G3582\uff09\u306f\u3001\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0\u5316\u5408\u7269MTS\u3092\u7740\u8272\u3055\u308c\u305f\u30db\u30eb\u30de\u30b6\u30f3\u7523\u7269\u306b\u9084\u5143\u3059\u308b\u7d30\u80de\u306e\u80fd\u529b\u3092\u6e2c\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u751f\u5b58\u5ea6\u306b\u3064\u3044\u3066\u306ein vitro \u306e\u60c5\u5831\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u6d3b\u767a\u306a\u7d30\u80de\u4ee3\u8b1d\u306b\u3088\u308a\u3001\u30c6\u30c8\u30e9\u30be\u30ea\u30a6\u30e0\u9084\u5143\u306b\u5fc5\u8981\u306aNADH\u306e\u3088\u3046\u306a\u9084\u5143\u7269\u8cea\u306e\u751f\u6210\u304c\u3082\u305f\u3089\u3055\u308c\u307e\u3059\u3002\u7740\u8272\u3055\u308c\u305f\u30db\u30eb\u30de\u30b6\u30f3\u7523\u7269\u3078\u306eMTS\u306e\u5909\u63db\u306e\u5897\u52a0\u306f\u3001\u751f\u5b58\u3059\u308b\u7d30\u80de\u6570\u306e\u76f8\u5bfe\u7684\u306a\u6307\u6a19\u3068\u306a\u308a\u3001\u6e1b\u5c11\u306f\u7d30\u80de\u6b7b\u306e\u76f8\u5bfe\u7684\u306a\u6307\u6a19\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u6700\u8fd1\u3001\u30ab\u30b9\u30d1\u30fc\u30bc-8\u306e1\u756a\u76ee\u306e\u6b7b\u4f5c\u52d5\u4f53\u30c9\u30e1\u30a4\u30f3\uff08DED\uff09\u304c\u305d\u306e\u30ab\u30b9\u30d1\u30fc\u30bc\u6d3b\u6027\u306b\u4f9d\u5b58\u305b\u305a\u306bc-Jun N-terminal kinase\uff08JNK\uff09\u7d4c\u8def\u3092\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3053\u3068\u3001\u305d\u3057\u3066\u540c\u3058\u304f\u3053\u306e\u3088\u3046\u306a\u6d3b\u6027\u5316\u3092\u963b\u6b62\u3059\u308b\u3053\u306e\u30c9\u30e1\u30a4\u30f3\u306b\u304a\u3051\u308b\u7a81\u7136\u5909\u7570\u304cFas\u30ec\u30bb\u30d7\u30bf\u30fc\u306b\u3088\u3063\u3066\u5a92\u4ecb\u3055\u308c\u308b\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u963b\u6b62\u3059\u308b\u3068\u3044\u3046\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u3055\u308c\u307e\u3057\u305f\uff088\uff09\u3002\u3057\u305f\u304c\u3063\u3066\u3001JNK \u306f\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306b\u304a\u3044\u3066\u3082\u4f55\u3089\u304b\u306e\u5f79\u5272\u3092\u6f14\u3058\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002JNK\u7d4c\u8def\u306e\u5206\u6790\u306e\u305f\u3081\u306b\u3001\u30d7\u30ed\u30e1\u30ac\u306fAnti-ACTIVE\u00ae JNK pAb, Rabbit \uff08\u30ab\u30bf\u30ed\u30b0\u756a\u53f7V7931\u3068V7932\uff09\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u3053\u306e\u6297\u4f53\u306f\u30a6\u30a7\u30b9\u30bf\u30f3\u5206\u6790\u3068\u514d\u75ab\u7d30\u80de\u67d3\u8272\u306e\u4e21\u65b9\u3067\u4f7f\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \uff08eNotes &#8211; FAQ)<\/p>\n\n\n\n<p><strong>\u95a2\u9023\u30ea\u30f3\u30af<\/strong>\uff1a<br><a href=\"http:\/\/www.promega.com\/resources\/product-guides-and-selectors\/protocols-and-applications-guide\/apoptosis\/#title2\">http:\/\/www.promega.com\/resources\/product-guides-and-selectors\/protocols-and-applications-guide\/apoptosis\/#title2<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u305f\u7d30\u80de\u6b7b\u306b\u5bfe\u3059\u308b\u8208\u5473\u306e\u9ad8\u307e\u308a\u306f\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306e\u591a\u304f\u306e\u624b\u6cd5\u306e\u767a\u9054\u3092\u3082\u305f\u3089\u3057\u307e\u3057\u305f\u3002\u30d7\u30ed\u30e1\u30ac\u306f\u3001Anti-ACTIVE\u00ae Caspase-3 pAb\u3001Anti-PARP p85 Fragment  &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[229],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/896"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=896"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/896\/revisions"}],"predecessor-version":[{"id":898,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/896\/revisions\/898"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=896"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=896"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=896"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}