{"id":915,"date":"2020-12-03T10:18:26","date_gmt":"2020-12-03T01:18:26","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=915"},"modified":"2021-01-19T11:33:34","modified_gmt":"2021-01-19T02:33:34","slug":"q497","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/reporter-signaling\/protein-reporter\/ppi\/nanobit\/q497","title":{"rendered":"NanoBRET\u2122\u3068\u306e\u9055\u3044\u3092\u6559\u3048\u3066\u4e0b\u3055\u3044"},"content":{"rendered":"\n

\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002NanoBiT\u2122 \u3068NanoBRET\u2122 \u306e\u7279\u6027\u6bd4\u8f03<\/p>\n\n\n\n

\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n
\u7279\u6027<\/th>\n NanoBRET\u2122<\/th>\n NanoBiT\u2122<\/th>\n <\/tr>\n
\u65b9\u5f0f<\/td>\n \u751f\u7269\u767a\u5149\u5171\u9cf4\u3000\u30a8\u30cd\u30eb\u30ae\u30fc\u8ee2\u79fb\uff08BRET\uff09<\/td>\n \u767a\u5149\u9175\u7d20\u65ad\u7247\u306e\u76f8\u88dc\u6027<\/td>\n <\/tr>\n
\u691c\u51fa\u5bfe\u8c61<\/td>\n \u8fd1\u63a5\u5ea6<\/td>\n \u76f4\u63a5\u7684\u306a\u76f8\u4e92\u4f5c\u7528<\/td>\n <\/tr>\n
\u30bf\u30b0\u306e\u5927\u304d\u3055<\/td>\n \u5c0f\u3055\u3044
20 kDa\uff08NanoLuc\u2122\uff09\u306830 kDa\uff08HaloTag\u00ae<\/sup>\uff09<\/td>\n 		\u975e\u5e38\u306b\u5c0f\u3055\u3044
11\u30a2\u30df\u30ce\u9178\uff08SmBiT\uff09\u3068<18 kDa\uff08LgBiT\uff09<\/td>\n <\/tr>\n
\u30c7\u30fc\u30bf<\/td>\n 2\u6ce2\u9577\u306e\u6bd4\u7387\uff08\u4f4e %CV\uff09<\/td>\n RLU\uff08\u76f8\u5bfe\u767a\u5149\u5f37\u5ea6\uff09<\/td>\n <\/tr>\n
\u8a66\u85ac\u6dfb\u52a0\u56de\u6570<\/td>\n 2<\/td>\n 1<\/td>\n <\/tr>\n
\u691c\u51fa\u6a5f\u5668<\/td>\n \u30a2\u30c3\u30bb\u30a4<\/td>\n BRET \u5bfe\u5fdc\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc
\uff08\u9069\u5408\u30d5\u30a3\u30eb\u30bf\u30fc\u8981\uff09<\/td>\n 		\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc
\uff08\u30d5\u30a3\u30eb\u30bf\u30fc\u4e0d\u8981\uff09<\/td>\n <\/tr>\n
\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0<\/td>\n \u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u30b7\u30b9\u30c6\u30e0
\uff08Olympus LV200 \u306a\u3069\uff09\uff1a
\u76f8\u4e92\u4f5c\u7528\u524d\u5f8c\u3092\u89b3\u5bdf\u53ef\u80fd<\/td>\n 		\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u30b7\u30b9\u30c6\u30e0
\uff08Olympus LV200 \u306a\u3069\uff09\uff1a
\u8907\u5408\u4f53\u306e\u307f\u89b3\u5bdf\u53ef\u80fd<\/td>\n <\/tr>\n
\u53ef\u9006\u6027
\uff08\u30ad\u30cd\u30c6\u30a3\u30c3\u30af\u30a2\u30c3\u30bb\u30a4\u53ef\uff09<\/td>\n 		\u25ef<\/td>\n \u25ef<\/td>\n <\/tr>\n
\u88fd\u54c1\u5f62\u614b<\/td>\n \u30ad\u30c3\u30c8\uff08100\u7a2e\u985e\u4ee5\u4e0a\uff09\u3001
\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u3001\u691c\u51fa\u8a66\u85ac<\/td>\n 		\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30d9\u30af\u30bf\u30fc\u3001
\u691c\u51fa\u8a66\u85ac<\/td>\n <\/tr>\n
\u30d9\u30af\u30bf\u30fc\u69cb\u7bc9\u30b5\u30fc\u30d3\u30b9\u3082\u3054\u3056\u3044\u307e\u3059\u3002<\/td>\n <\/tr>\n
\u30e9\u30a4\u30bb\u30f3\u30b9\u8cbb\u7528\uff08\u4f01\u696d\u3092\u542b\u3080\uff09<\/td>\n \u8a66\u85ac\u306e\u8cfc\u5165\u306e\u307f<\/td>\n <\/tr>\n
\u305d\u306e\u4ed6<\/td>\n HaloTag\u5074\u3092\u4f7f\u3063\u305f\u30d7\u30eb\u30c0\u30a6\u30f3<\/td>\n <\/td>\n <\/tr>\n <\/tbody>\n <\/table>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002NanoBiT\u2122 \u3068NanoBRET\u2122 \u306e\u7279\u6027\u6bd4\u8f03 \u7279\u6027 NanoBRET\u2122 NanoBiT\u2122 \u65b9\u5f0f \u751f\u7269\u767a\u5149\u5171\u9cf4\u3000\u30a8\u30cd\u30eb\u30ae\u30fc\u8ee2\u79fb\uff08BRET\uff09 \u767a\u5149\u9175\u7d20\u65ad\u7247\u306e\u76f8\u88dc\u6027 \u691c\u51fa\u5bfe\u8c61 \u8fd1\u63a5\u5ea6 \u76f4\u63a5\u7684\u306a\u76f8\u4e92 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[197],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/915"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=915"}],"version-history":[{"count":11,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/915\/revisions"}],"predecessor-version":[{"id":2215,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/915\/revisions\/2215"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=915"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=915"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=915"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}