{"id":917,"date":"2020-12-03T10:18:19","date_gmt":"2020-12-03T01:18:19","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=917"},"modified":"2021-05-14T15:35:03","modified_gmt":"2021-05-14T06:35:03","slug":"q725","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/cytotoxity\/caspase\/caspase-glo\/q725","title":{"rendered":"\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u304c\u8a98\u5c0e\u3055\u308c\u3066\u3044\u306a\u3044\u7d30\u80de\uff08Background\uff09\u3067\u6e2c\u5b9a\u5024\u304c\u9ad8\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002\u3069\u306e\u3088\u3046\u306a\u539f\u56e0\u304c\u8003\u3048\u3089\u308c\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n

Caspase-Glo\u00ae Assay\u306f\u611f\u5ea6\u306e\u9ad8\u3044\u88fd\u54c1\u3067\u3059\u306e\u3067\u3001\u5c11\u306a\u3044\u7d30\u80de\u6570\u3067\u3082Caspase\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u304c\u8a98\u5c0e\u3055\u308c\u3066\u3044\u306a\u3044\u7d30\u80de\u3067\u6e2c\u5b9a\u5024\u304c\u9ad8\u304f\u306a\u3063\u3066\u3057\u307e\u3046\u8981\u56e0\u306f\u3001\u4ee5\u4e0b\u306e2\u70b9\u306b\u3064\u3044\u3066\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

1) well\u3042\u305f\u308a\u306b\u542b\u307e\u308c\u308b\u7d30\u80de\u6570\u304c\u591a\u304f\u3001\u30b3\u30f3\u30d5\u30eb\u30a8\u30f3\u30c8\u306a\u72b6\u614b\u306b\u306a\u3063\u3066\u3044\u308b
2) \u57f9\u5730\u6210\u5206\u306bLuciferase\u306e\u53cd\u5fdc\u306b\u5f71\u97ff\u3059\u308b\u7269\u8cea\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3002
\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u306f\u3001\u523a\u6fc0\u3092\u52a0\u3048\u3066\u3044\u306a\u3044\u7d30\u80de\uff08Background\uff09\u3068\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u78ba\u5b9f\u306b\u8a98\u5c0e\u3067\u304d\u308b\u7269\u8cea\u3067\u51e6\u7406\u3057\u305f\u7d30\u80de\uff08Positive control\uff1b\u5b9f\u9a13\u7cfb\u306f\u88681\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\uff09\u3067\u78ba\u8a8d\u3044\u305f\u3060\u3044\u3066\u304b\u3089\u3001\u6e2c\u5b9a\u30fb\u6bd4\u8f03\u3092\u3057\u3066\u304f\u3060\u3055\u3044\uff08\u56f31\uff09\u3002<\/p><\/blockquote>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56f31\u3000Jurkat cells \u3092anti-Fas mAb \u3067 4.5 \u6642\u9593\u51e6\u7406\u3057\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u8a98\u5c0e\u3055\u305b\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001Caspase-Glo\u00ae 3\/7\u8a66\u85ac\u3092\u6dfb\u52a0\u3057\u30011\u6642\u9593\u5f8c\u306b\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u6e2c\u5b9a\u3057\u307e\u3057\u305f\u3002 96 well plate\u3067\u306e\u53cd\u5fdc\u5bb9\u91cf\u306f200\u03bcL\u3067\u3059\uff08\u3046\u3061\u3001\u6dfb\u52a0\u3057\u305fCaspase-Glo\u00ae\u8a66\u85ac\u306f100\u03bcL\u3067\u3059\uff09\u3002<\/p><\/blockquote>\n\n\n\n

\u88681\u3000\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u8a98\u5c0e\u306e\u6761\u4ef6<\/p>\n\n\n\n

\n \n \n \n \n \n \n \n \n \n \n \n \n \n
Caspase<\/th>\n \u7d30\u80de\u7a2e<\/th>\n \u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u8a98\u5c0e\u7269\u8cea<\/th>\n \u6fc3\u5ea6\uff08\u7d42\u6fc3\u5ea6\uff09<\/th>\n \u8a98\u5c0e\u6642\u9593<\/th>\n <\/tr>\n
Caspase-3\/7<\/td>\n Jurkat \uff0810,000 cells\/well\uff09<\/td>\n Anti-Fas \u6297\u4f53<\/td>\n 100-200ng\/mL<\/td>\n 4-5 \u6642\u9593<\/td>\n <\/tr>\n
Caspase-3\/7<\/td>\n Staurosporine<\/td>\n 500-1000nM<\/td>\n 4-5\u6642\u9593<\/td>\n <\/tr>\n
Caspase-3\/7<\/td>\n HeLa \uff0810,000 cells\/well\uff09<\/td>\n Staurosporine<\/td>\n 1000nM<\/td>\n 5 \u6642\u9593<\/td>\n <\/tr>\n
Caspase-3\/7<\/td>\n MCF-7 (25,000 cells\/well)<\/td>\n Staurosporine<\/td>\n 5\u03bcM<\/td>\n 5 \u6642\u9593<\/td>\n <\/tr>\n
Caspase-3\/7<\/td>\n Rat Primary Striatal Neural Culture\uff0825,000 cells\/well\uff09<\/td>\n Staurosporine<\/td>\n 3-5\u03bcM<\/td>\n 5 \u6642\u9593<\/td>\n <\/tr>\n
Caspase-3\/7<\/td>\n HepG2 variant \uff0840,000 cells\/well\uff09<\/td>\n Staurosporine<\/td>\n 500-1000nM<\/td>\n 24 \u6642\u9593<\/td>\n <\/tr>\n
Caspase-3\/7<\/td>\n PC 12 \uff0810,000-20,000 cells \/well\uff09<\/td>\n Staurosporine<\/td>\n 10\u03bcM<\/td>\n 5\u6642\u9593\uff5e10\u6642\u9593<\/td>\n <\/tr>\n
Caspase-8<\/td>\n Jurkat \uff0810,000-50,000 cells \/well\uff09<\/td>\n Anti-Fas \u6297\u4f53<\/td>\n 100 ng\/mL<\/td>\n 3\u6642\u9593<\/td>\n <\/tr>\n
Caspase-9<\/td>\n HL-60 \uff0810,000-50,000 cells \/well\uff09<\/td>\n Vinblastine<\/td>\n 50\u03bcM<\/td>\n 3\u6642\u9593<\/td>\n <\/tr>\n
Caspase-8, 9<\/td>\n HeLa \uff0825,000 cells \/well\uff09<\/td>\n Staurosporine<\/td>\n 50\u03bcM<\/td>\n 0\uff5e5\u6642\u9593\u51e6\u7406\u3057\u300130\u5206\u9593\u9694\u3067\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3063\u305f\u7d50\u679c\u30012.5\u6642\u9593 \u304b\u3089\u6d3b\u6027\u304c\u691c\u51fa\u3055\u308c\u30014.5\u6642\u9593\u3067\u6700\u5927\u6d3b\u6027\u304c\u8a8d\u3081\u3089\u308c\u307e\u3059\u3002<\/td>\n <\/tr>\n <\/tbody>\n <\/table>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"

Caspase-Glo\u00ae Assay\u306f\u611f\u5ea6\u306e\u9ad8\u3044\u88fd\u54c1\u3067\u3059\u306e\u3067\u3001\u5c11\u306a\u3044\u7d30\u80de\u6570\u3067\u3082Caspase\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u304c\u8a98\u5c0e\u3055\u308c\u3066\u3044\u306a\u3044\u7d30\u80de\u3067\u6e2c\u5b9a\u5024\u304c\u9ad8\u304f\u306a\u3063\u3066\u3057\u307e\u3046\u8981\u56e0\u306f\u3001\u4ee5\u4e0b\u306e2\u70b9\u306b\u3064\u3044\u3066\u8003\u3048\u3089\u308c\u307e\u3059\u3002 … <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[230],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/917"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=917"}],"version-history":[{"count":9,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/917\/revisions"}],"predecessor-version":[{"id":2892,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/917\/revisions\/2892"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=917"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=917"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=917"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}