{"id":926,"date":"2020-12-03T10:19:50","date_gmt":"2020-12-03T01:19:50","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=926"},"modified":"2021-01-20T11:40:03","modified_gmt":"2021-01-20T02:40:03","slug":"q728","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/cytotoxity\/caspase\/caspase-glo\/q728","title":{"rendered":"\u7d44\u7e54\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u3044\u3066Caspase\u3092\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001Caspase-Glo\u2122\u3092\u7528\u3044\u3066\u7d44\u7e54\u306b\u5b58\u5728\u3059\u308bCaspase\u6d3b\u6027\u306f\u78ba\u8a8d\u3057\u3066\u304a\u308a\u307e\u305b\u3093\u3002\u305f\u3060\u3057\u3001\u6587\u732e<sup>\uff081\uff09<\/sup>\u3067\u30de\u30a6\u30b9\u306e\u809d\u81d3\u3092\u30db\u30e2\u30b8\u30cd\u30fc\u30c8\u3057\u305f\u306e\u3061\u3001Caspase-Glo\u2122\u3092\u7528\u3044\u3066Caspase-3\/7, 8, 9\u3092\u6e2c\u5b9a\u3057\u305f\u5831\u544a\u4f8b\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u8ad6\u6587\u306e\u624b\u9806\u306f\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote\"><p>1) \u30de\u30a6\u30b9\u306e\u7d44\u7e54\u3092\u4f4e\u6d78\u900f\u6027buffer(25 mM HEPES, pH 7.5, 5 mM MgCl<sub>2<\/sub>, 1 mM EGTA, 1 mM Pefablock, 1 \u03bcg\/mL, pepstatin,\u3000leupeptin, aprotinin)\u3067\u30db\u30e2\u30b8\u30cd\u30fc\u30c8\u3059\u308b<br>2) 13,000rpm\u30014 \u2103\u300115\u5206\u9593\u9060\u5fc3<br>3) \u30bf\u30f3\u30d1\u30af\u6fc3\u5ea6\u30921mg\/mL\u3068\u306a\u308b\u3088\u3046\u306b\u8abf\u6574\u3059\u308b<br>4) 10\u03bcg\/mL\u306e\u7d30\u80de\u8cea\u30bf\u30f3\u30d1\u30af\u3068\u540c\u3058\u5bb9\u91cf\u306eCaspase-Glo\u2122\u8a66\u85ac\u309296 well plate\u306b\u52a0\u3048\u3001\u5ba4\u6e29\u30671\u6642\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8<br>5) \u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u767a\u5149\u5024\u3092\u6e2c\u5b9a<\/p><p>\u53c2\u8003\u6587\u732e\uff081\uff09:<br><em>Liu, D, Li, C, Chen, Y, Burnett, C, Liu, XY, Downs, S, Collins, RD, and Hawiger, J.Nuclear Import of Proinflammatory Transcription Factors Is Required for Massive Liver Apoptosis Induced by Bacterial Lipopolysaccharide.\u3000Journal of Biological Chemistry 279, (46) pp. 48434-48442, 2004<\/em><\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>\u30d7\u30ed\u30e1\u30ac\u3067\u306f\u3001Caspase-Glo\u2122\u3092\u7528\u3044\u3066\u7d44\u7e54\u306b\u5b58\u5728\u3059\u308bCaspase\u6d3b\u6027\u306f\u78ba\u8a8d\u3057\u3066\u304a\u308a\u307e\u305b\u3093\u3002\u305f\u3060\u3057\u3001\u6587\u732e\uff081\uff09\u3067\u30de\u30a6\u30b9\u306e\u809d\u81d3\u3092\u30db\u30e2\u30b8\u30cd\u30fc\u30c8\u3057\u305f\u306e\u3061\u3001Caspase-Glo\u2122\u3092\u7528\u3044\u3066Caspase-3\/7, 8,  &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[230],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/926"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=926"}],"version-history":[{"count":1,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/926\/revisions"}],"predecessor-version":[{"id":927,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/926\/revisions\/927"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=926"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=926"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=926"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}