{"id":954,"date":"2020-12-03T10:27:40","date_gmt":"2020-12-03T01:27:40","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=954"},"modified":"2021-01-20T11:50:16","modified_gmt":"2021-01-20T02:50:16","slug":"q737","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/cytotoxity\/tunel\/dead-end\/q737","title":{"rendered":"DeadEnd\u2122 Colorimetric Apoptosis Detection System\u3092\u4f7f\u3046\u3068\u304d\u306b\u6700\u9069\u5316\u3059\u308b\u306e\u304c\u5fc5\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u306f\u4f55\u3067\u3059\u304b\u3002"},"content":{"rendered":"\n<p>DeadEnd\u2122 System\u3092\u4f7f\u3063\u3066\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u89e3\u6790\u3059\u308b\u6642\u306b\u30ad\u30fc\u3068\u306a\u308b\u8981\u56e0\u306f\u7d30\u80de\u3084\u7d44\u7e54\u3092\u6d78\u900f\u5316\u3059\u308b\u305f\u3081\u306e\u65b9\u6cd5\u3067\u3059\u3002\u30c1\u30e3\u30f3\u30d0\u30fc\u30b9\u30e9\u30a4\u30c9\u3084poly-L-lysine\u3067\u30b3\u30fc\u30c8\u3057\u305f\u30b9\u30e9\u30a4\u30c9\u306e\u4e0a\u3067\u57f9\u990a\u3055\u308c\u305f\u7d30\u80de\u3067\u306f\u300110% buffered formalin\u30844% paraformaldehyde\u306e\u8fc5\u901f\u306a\u56fa\u5b9a\u5316\u306b\u7d9a\u304f\u30010.2% Triton X-100\u306e\u6d78\u900f\u5316\u304c\u4e00\u822c\u7684\u306b\u9069\u5f53\u3067\u3059\u3002\u3057\u304b\u3057\u7d44\u7e54\u3067\u306f\u3088\u308a\u53b3\u5bc6\u306a\u65b9\u6cd5\u304c\u8981\u6c42\u3055\u308c\u307e\u3059\u3002DeadEnd\u2122 System\u306f\u3001\u7d44\u7e54\u306e\u6d78\u900f\u5316\u306e\u305f\u3081\u306bProteinase K\u3092\u6dfb\u4ed8\u3057\u3066\u3044\u307e\u3059\u3002\u7d44\u7e54\u306e\u5207\u7247\u306f\u300120\u03bcg\/ml Proteinase K\u306710\uff5e30\u5206\u9593\u5ba4\u6e29\u3067\u51e6\u7406\u3057\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u8584\u3044\u5207\u7247\uff08\u4f8b\u3048\u30705\uff5e10um\u306e\u30d1\u30e9\u30d5\u30a3\u30f3\u5207\u7247\uff09\u3067\u306f\u77ed\u3044\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u6642\u9593\u3067\u5341\u5206\u3067\u3059\u3002\u4e00\u65b9\u3001\u539a\u3044\u5207\u7247\uff08\u4f8b\u3048\u307050\u03bcm\u306e\u30d3\u30d6\u30e9\u30c8\u30fc\u30e0\u5207\u7247\uff09\u3067\u306f\u9577\u3044\u6642\u9593\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u9577\u6642\u9593\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30b7\u30e7\u30f3\u306b\u3088\u3063\u3066\u30b9\u30e9\u30a4\u30c9\u304b\u3089\u7d44\u7e54\u5207\u7247\u304c\u306f\u304c\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306e\u8a98\u5c0e\u7269\u8cea\u3084\u5b9f\u9a13\u306e\u6761\u4ef6\u3092\u8a2d\u5b9a\u3059\u308b\u305f\u3081\u306e\u4e88\u5099\u5b9f\u9a13\u3067\u306f\u3001\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u5b9f\u9a13\u304c\u5fc5\u9808\u3067\u3059\u3002\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3092\u6e96\u5099\u3059\u308b\u305f\u3081\u306b\u3001\u7d30\u80de\u3084\u7d44\u7e54\u5207\u7247\u3092\u6d78\u900f\u5316\u3057\u305f\u5f8c\u3001DNaseI\u3092\u30b9\u30e9\u30a4\u30c9\u306b\u52a0\u3048\u307e\u3059\u3002\u65ad\u7247\u5316\u3055\u308c\u305f\u30b2\u30ce\u30e0DNA\u306f\u3001TdT\u306b\u3088\u3063\u3066\u30e9\u30d9\u30eb\u3055\u308c\u307e\u3059\u3002\u3053\u306e\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306fDeadEnd\u2122 System\u306b\u6dfb\u4ed8\u3055\u308c\u308b\u53d6\u6271\u8aac\u660e\u66f8\uff08Technical Bulletin\uff09\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\uff081\uff09\u3002\u6a19\u6e96\u7684\u306a\u8a66\u9a13\u3067\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u3092\u8a98\u5c0e\u3059\u308b\u3068\u63a8\u5b9a\u3055\u308c\u308b\u56e0\u5b50\u3067\u51e6\u7406\u3057\u305f\u7d30\u80de\u304c\u6a19\u8b58\u3055\u308c\u305a\u3001\u967d\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u304c\u967d\u6027\u3060\u3063\u305f\uff08\u6a19\u8b58\u3055\u308c\u305f\uff09\u5834\u5408\u3001\u30ad\u30c3\u30c8\u81ea\u4f53\u306f\u6b63\u3057\u304f\u6a5f\u80fd\u3057\u3066\u304a\u308a\u3001\u5168\u3066\u306e\u4e88\u6e2c\u3055\u308c\u308b\u53ef\u80fd\u6027\u306b\u304a\u3044\u3066\u3001\u30c6\u30b9\u30c8\u3055\u308c\u3066\u3044\u308b\u56e0\u5b50\u306f\u3053\u308c\u3089\u306e\u6761\u4ef6\u4e0b\u3067DNA\u306e\u65ad\u7247\u5316\u3092\u8a98\u5c0e\u3057\u3066\u3044\u306a\u3044\u3053\u3068\u306b\u306a\u308a\u307e\u3059\uff08\u3092\u793a\u3057\u3066\u3044\u307e\u3059\uff09\u3002\uff08PN72-Q&amp;A)<\/p>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e<\/strong>\uff1a<br>&nbsp;&nbsp;1. 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