{"id":964,"date":"2020-12-03T10:46:34","date_gmt":"2020-12-03T01:46:34","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=964"},"modified":"2021-05-10T16:18:37","modified_gmt":"2021-05-10T07:18:37","slug":"q741","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/adme\/p450\/p450-glo\/q741","title":{"rendered":"P450-Glo\u2122 Assay\u3068\u306f\u3069\u306e\u3088\u3046\u306a\u88fd\u54c1\u3067\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>P450-Glo\u2122 Assay\u306f\u3001\u30b7\u30c8\u30af\u30ed\u30e0P450\uff08\u4ee5\u4e0bCYP450\u3068\u7565\u3057\u307e\u3059\uff09\u306b\u5bfe\u3059\u308b\u963b\u5bb3\u5264\u3084\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u5316\u5408\u7269\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u76ee\u7684\u3067\u958b\u767a\u3055\u308c\u305f\u88fd\u54c1\u3067\u3059\u3002<br>CYP450\u7cbe\u88fd\u9175\u7d20\u3068\u4efb\u610f\u306e\u5316\u5408\u7269\u3092\u7528\u610f\u3057\u3001Luciferin\u8a98\u5c0e\u4f53\u3067\u3042\u308bP450-Glo\u2122 Luciferin Substrate \uff08\u88681\u53c2\u7167\uff09\u3068\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\uff08Step1\uff09\u3002\u3053\u3053\u3067\u53cd\u5fdc\u304c\u8d77\u3053\u308b\u3068\u3001 P450-Glo\u2122 Luciferin Substrate\u306f\u4ee3\u8b1d\u3055\u308c\u3066\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u57fa\u8cea\u3067\u3042\u308bLuciferin\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u6b21\u306b\u3001Luciferin Detection Reagent\uff08\u30b7\u30b9\u30c6\u30e0\u4ed8\u5c5e\u54c1\uff09\u3092\u52a0\u3048\u3066\u3001\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u53cd\u5fdc\u3092\u884c\u3044\u3001\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\u3067\u305d\u306e\u767a\u5149\u91cf\uff08RLU\u5024\uff09\u3092\u6e2c\u5b9a\u3057\u307e\u3059\uff08Step2\uff09\u6ce8\uff09\u3002<\/p>\n\n\n\n<p> Step1, 2\u306e\u6982\u7565\u56f3\u306f<a rel=\"noreferrer noopener\" href=\"https:\/\/www.promega.co.jp\/resources\/faq\/wp-content\/uploads\/4126MA05_3A.jpg\" target=\"_blank\">\u3053\u3061\u3089<\/a>\u3002<\/p>\n\n\n\n<p>CYP450\u306e\u6d3b\u6027\u304c\u9ad8\u3044\u3068\u3001RLU\u5024\u306f\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002\u9006\u306bCYP450\u306e\u6d3b\u6027\u304c\u4f4e\u3044\u3068\u3001RLU\u5024\u306f\u4f4e\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u4ee5\u4e0b\u306bP450-Glo\u2122 Luciferin Substrate\u3068\u305d\u308c\u306b\u5bfe\u5fdc\u3059\u308bCYP450\u306e\u5206\u5b50\u7a2e\u306e\u8868\u3092\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>\u88681\u3000P450-Glo\u2122 Assay\u3067\u4f7f\u7528\u3059\u308bP450-Glo\u2122 Luciferin Substrate\u3068<br>\u305d\u308c\u306b\u5bfe\u5fdc\u3059\u308bCYP450\u5206\u5b50\u7a2e<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><th>P450-Glo\u2122\u3000Luciferin\u3000Substrate<\/th><th><strong>CYP450\u5206\u5b50\u7a2e\u203b1<\/strong><\/th><th><strong>\u30a2\u30c3\u30bb\u30a4\u30bf\u30a4\u30d7<\/strong><\/th><\/tr><tr><td>Luciferin-ME<\/td><td><strong>1A2, 2C8,&nbsp;<\/strong>2C9, 2J2, 4A11, 4F3B, 19<\/td><td>\u9175\u7d20<\/td><\/tr><tr><td>Luciferin-1A2\u203b3<\/td><td><strong>1A2<\/strong><\/td><td>\u9175\u7d20\u3001\u751f\u7d30\u80de\u203b2<\/td><\/tr><tr><td>Luciferin-CEE<\/td><td><strong>1A1, 1B1,&nbsp;<\/strong>3A7<\/td><td>\u9175\u7d20\u3001\u751f\u7d30\u80de\u203b2<\/td><\/tr><tr><td>Luciferin-2B6\u203b3<\/td><td><strong>2B6<\/strong><\/td><td>\u9175\u7d20\u3001\u751f\u7d30\u80de\u203b2<\/td><\/tr><tr><td>Luciferin-H<\/td><td><strong>2C9<\/strong><\/td><td>\u9175\u7d20\u3001\u751f\u7d30\u80de\u203b2<\/td><\/tr><tr><td>Luciferin-ME EGE<\/td><td><strong>2D6<\/strong>, 1A1, 1A2, 2B6\u203b5<\/td><td>\u9175\u7d20<\/td><\/tr><tr><td>Luciferin-H EGE<\/td><td><strong>2C19<\/strong>, 1A1, 1A2<\/td><td>\u9175\u7d20<\/td><\/tr><tr><td>Luciferin-BE\u203b4<\/td><td><strong>3A4, 3A5, 3A7,&nbsp;<\/strong>4F12<\/td><td>\u9175\u7d20<\/td><\/tr><tr><td>Luciferin-PFBE\u203b4<\/td><td><strong>3A4,&nbsp;<\/strong>3A5, 3A7<\/td><td>\u9175\u7d20\u3001\u751f\u7d30\u80de\u203b2<\/td><\/tr><tr><td>Luciferin-PPXE\u203b4<\/td><td><strong>3A4,&nbsp;<\/strong>3A5, 3A7<\/td><td>\u9175\u7d20<\/td><\/tr><tr><td>Luciferin-IPA\u203b4<\/td><td><strong>3A4<\/strong><\/td><td>\u9175\u7d20\u3001\u751f\u7d30\u80de\u203b2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u6ce8\uff09\u6e2c\u5b9a\u306f\u3001<a href=\"https:\/\/www.promega.jp\/products\/microplate-readers-fluorometers-luminometers\/microplate-luminometers\/\">\u30eb\u30df\u30ce\u30e1\u30fc\u30bf\u30fc\uff08\u767a\u5149\u6e2c\u5b9a\u88c5\u7f6e\uff09<\/a>\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<br>\u203b1 \u592a\u5b57\u540d\u306e\u30ad\u30c3\u30c8\u7fa4\u306b\u3001\u8a72\u5f53Luciferin\u57fa\u8cea\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u88fd\u54c1\u304c\u3042\u308a\u307e\u3059\u3002<br>\u203b2 \u751f\u7d30\u80de\u3067\u306e\u30a2\u30c3\u30bb\u30a4\u306b\u3064\u3044\u3066\u306f<a rel=\"noreferrer noopener\" href=\"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/adme\/p450\/p450-glo\/q750\" target=\"_blank\">\u3053\u3061\u3089<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<br>\u203b3 CYP1A2\/Luciferin-1A2\u304a\u3088\u3073CYP2B6\/Luciferin-2B6\u306e\u4ee3\u8b1d\u7523\u7269\u306f\u30eb\u30b7\u30d5\u30a7\u30ea\u30f3\u524d\u99c6\u4f53\u3067\u3059\u3002Luciferin Detection Reagent\u4e2d\u306eD-Cysteine\u3068\u306e\u53cd\u5fdc\u306b\u3088\u308aD-luciferin\u304c\u751f\u6210\u3057\u307e\u3059\u3002<br>\u203b4 Luciferin-BE\u3001Luciferin-PFBE\u3001Luciferin-PPXE\u3001Luciferin-IPA\u306e\u4f7f\u3044\u5206\u3051\u306b\u95a2\u3057\u3066\u306f\u3001<a rel=\"noreferrer noopener\" href=\"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/adme\/p450\/p450-glo\/q754\" target=\"_blank\">\u3053\u3061\u3089<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<br>\u203b5 \u53cd\u5fdc\u7cfb\u306bcytochrome b5\u304c\u3042\u308b\u5834\u5408\u3001CYP2B6\u6d3b\u6027\u306f\u691c\u51fa\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>P450-Glo\u2122 Assay\u306f\u3001\u30b7\u30c8\u30af\u30ed\u30e0P450\uff08\u4ee5\u4e0bCYP450\u3068\u7565\u3057\u307e\u3059\uff09\u306b\u5bfe\u3059\u308b\u963b\u5bb3\u5264\u3084\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u5316\u5408\u7269\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u76ee\u7684\u3067\u958b\u767a\u3055\u308c\u305f\u88fd\u54c1\u3067\u3059\u3002CYP450\u7cbe\u88fd\u9175\u7d20\u3068\u4efb\u610f\u306e\u5316\u5408\u7269\u3092\u7528\u610f\u3057\u3001Lucifer &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[235],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/964"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=964"}],"version-history":[{"count":7,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/964\/revisions"}],"predecessor-version":[{"id":2793,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/964\/revisions\/2793"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=964"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=964"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}