{"id":983,"date":"2020-12-03T10:47:40","date_gmt":"2020-12-03T01:47:40","guid":{"rendered":"https:\/\/www.promega.co.jp\/resources\/faq\/?p=983"},"modified":"2021-09-21T13:14:05","modified_gmt":"2021-09-21T04:14:05","slug":"q744","status":"publish","type":"post","link":"https:\/\/www.promega.co.jp\/resources\/faq\/cell-health\/adme\/p450\/p450-glo\/q744","title":{"rendered":"\u30ad\u30c3\u30c8\u4ee5\u5916\u306b\u7528\u610f\u3059\u308b\u8a66\u85ac\u306a\u3069\u306f\u3042\u308a\u307e\u3059\u304b\uff1f"},"content":{"rendered":"\n<p>P450-Glo\u2122 Assay System\u3092\u4f7f\u7528\u3057\u3066\u9175\u7d20\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3046\u5834\u5408\u3001\u4ee5\u4e0b\u306e\u8a66\u85ac\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<br><a rel=\"noreferrer noopener\" href=\"https:\/\/www.promega.jp\/Resources\/Protocols\/Technical%20Bulletins\/101\/P450%20Glo%20Assays%20Protocol\/\" target=\"_blank\">\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb<\/a>\u300116\u30da\u30fc\u30b8\u306e\u300c\u304a\u5ba2\u69d8\u304c\u5225\u9014\u3054\u7528\u610f\u3044\u305f\u3060\u304f\u3082\u306e\u300d\u306b\u8a18\u8f09\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<ul><li>1M KPO<sub>4<\/sub>&nbsp;(pH7.4)\u3000\uff08\u7d44\u6210\u306f\u3001\u30de\u30cb\u30e5\u30a2\u30eb47\u30da\u30fc\u30b8\u306b\u3042\u308a\u307e\u3059\uff09<\/li><li>\u6ec5\u83cc\u6c34\u307e\u305f\u306f\u8131\u30a4\u30aa\u30f3\u6c34<\/li><li>CYP450\u6d3b\u6027\u306e\u3042\u308b\u8a66\u85ac\uff08\u7cbe\u88fd\u9175\u7d20\u306a\u3069\uff09\u3001CYP450\u9084\u5143\u9175\u7d20\u3092\u542b\u3080\u3082\u306e<br>CYP2C8\u30012C9\u30013A4\u30013A5\u30013A7\u306e\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3046\u5834\u5408\u306f\u3001CYP450\u9084\u5143\u9175\u7d20\u306b\u52a0\u3048\u3066cytochrome b5\u6d3b\u6027\u3082\u542b\u3080\u3082\u306e\u3092\u63a8\u5968\u3057\u307e\u3059\u3002<br>CYP450\u7cbe\u88fd\u9175\u7d20\u306f\u30b3\u30fc\u30cb\u30f3\u30b0\u30a4\u30f3\u30bf\u30fc\u30ca\u30b7\u30e7\u30ca\u30eb\u682a\u5f0f\u4f1a\u793e\uff08\u65e7BD Gentest\u793e\u88fd\uff09\u3001\u65e5\u672c\u8fb2\u7523\u682a\u5f0f\u4f1a\u793e\uff08Cypex\u793e\u88fd\uff09\u306a\u3069\u8907\u6570\u306e\u4f1a\u793e\u3067\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30de\u30cb\u30e5\u30a2\u30eb47\u30da\u30fc\u30b8\u306b\u8a73\u7d30\u306a\u60c5\u5831\u304c\u3042\u308a\u307e\u3059\u3002<\/li><li>CYP450\u6d3b\u6027\u3092\u6b20\u640d\u3059\u308b\u8a66\u85ac<br>minus-P450 control reaction\u306b\u4f7f\u7528\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001CYP450\u6d3b\u6027\u306e\u3042\u308b\u8a66\u85ac\u3068\u3057\u3066CYP450\u30ea\u30b3\u30f3\u30d3\u30ca\u30f3\u30c8\u9175\u7d20\u3092\u4f7f\u3046\u5834\u5408\u306f\u3001CYP450\u3092\u767a\u73fe\u3055\u305b\u3066\u3044\u306a\u3044control membrane\u306a\u3069\u3092\u304a\u4f7f\u3044\u4e0b\u3055\u3044\u3002<\/li><li>NADPH Regenerating System\uff1a<br>Promega, NADPH Regeneration System (Cat# V9510) <br>\u3000V9510\u306e\u69cb\u6210\u54c1<br>\u3000NADPH Regeneration System Solution A, 2.75mL<br>\u3000NADPH Regeneration System Solution B, 0.60mL<br>\u3000\u305d\u308c\u305e\u308c\u306e\u8a66\u85ac\u306e\u7d44\u6210\u306f\u30de\u30cb\u30e5\u30a2\u30eb47\u30da\u30fc\u30b8\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br>Promega \u4ee5\u5916\u3067\u306f\u3001\u4ee5\u4e0b\u306e\u30e1\u30fc\u30ab\u30fc\u304b\u3089\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br>1) \u30b3\u30fc\u30cb\u30f3\u30b0\u30a4\u30f3\u30bf\u30fc\u30ca\u30b7\u30e7\u30ca\u30eb\u682a\u5f0f\u4f1a\u793e\uff08\u65e7BD Gentest\u793e\u88fd\uff09<br>\u3000NADPH Regeneration System Solution A (Cat#451220, 5mL)<br>\u3000NADPH Regeneration System Solution B (Cat#451200, 1mL)<br>2) Sigma \uff08\u4e0b\u8a18\u306e\u8a66\u85ac\u3092\u4f7f\u7528\u3057\u3066\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002\uff09<br>\u3000G-6-P-dehydrogenase from baker&#8217;s yeast (Cat# G6378, 100units)<br>\u3000G-6-P disodium salt hydrate (Cat# G7250, 500mg)<br>\u3000NADP sodium salt (Cat# N0505, 100mg)<\/li><li>\u30a2\u30bb\u30c8\u30cb\u30c8\u30ea\u30eb\uff08CYP2D6\u30012C19\u30a2\u30c3\u30bb\u30a4\u306e\u307f\uff09<\/li><li>100mM Tris-HCl, pH7.5\uff08Luciferin-PPXE\u3092\u7528\u3044\u305fCYP3A4\u30a2\u30c3\u30bb\u30a4\u306e\u307f\uff09<\/li><\/ul>\n\n\n\n<p>&lt;\u53c2\u8003&gt;<br>P450-Glo\u2122 CYP450 Screening System (CYP1A2 Cat#V9770, CYP2B6 Cat#V9781, CYP2C9 Cat#V9790, CYP3A4 Cat# V9800\u3001V9910\u304a\u3088\u3073V9920, CYP2C19 Cat# V9880, CYP2D6 Cat# V9890, \u54041,000\u56de\u5206)\u306b\u3064\u3044\u3066\u306f\u3001\u4e0a\u8a18\u306e\u8a66\u85ac\u304c\u5168\u3066\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u4ed8\u5c5e\u306eCYP450\u7cbe\u88fd\u9175\u7d20\u306f\u3001\u6606\u866b\u7d30\u80de\u3067\u767a\u73fe\u3055\u305b\u305f\u30d2\u30c8CYP450\u306e\u7d44\u63db\u3048\u4f53\u3067\u3059\u3002\u307e\u305f\u3001\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30e1\u30f3\u30d6\u30ec\u30f3\uff085mg\/mL, 100\u03bcL\uff09\u3082\u4ed8\u5c5e\u54c1\u3068\u3057\u3066\u6dfb\u4ed8\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u3061\u3089\u306f\u3001CYP450\u7cbe\u88fd\u9175\u7d20\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u3001CYP450\u7cbe\u88fd\u9175\u7d20\u306e\u4ee3\u308f\u308a\u306b\u3001\u30c6\u30b9\u30c8\u30b5\u30f3\u30d7\u30eb\u3067\u4f7f\u7528\u3059\u308bCYP450\u7cbe\u88fd\u9175\u7d20\u3068\u7b49\u91cf\u3092\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p>P450-Glo\u2122 CYP450 Screening System\u3000<a href=\"https:\/\/www.promega.jp\/Resources\/Protocols\/Technical%20Bulletins\/101\/P450%20Glo%20Assays%20Protocol\/\" target=\"_blank\" rel=\"noreferrer noopener\">\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb<\/a><\/p>\n\n\n\n<p>D-Luciferin Standard Curve\u3092\u4f5c\u6210\u3059\u308b\u5834\u5408\u306f\u3001D-Luciferin\uff08Beetle Luciferin, Potassium Salt, Cat# E1601\u306a\u3069\uff09\u304c\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>P450-Glo\u2122 Assay System\u3092\u4f7f\u7528\u3057\u3066\u9175\u7d20\u30a2\u30c3\u30bb\u30a4\u3092\u884c\u3046\u5834\u5408\u3001\u4ee5\u4e0b\u306e\u8a66\u85ac\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u88fd\u54c1\u30de\u30cb\u30e5\u30a2\u30eb\u300116\u30da\u30fc\u30b8\u306e\u300c\u304a\u5ba2\u69d8\u304c\u5225\u9014\u3054\u7528\u610f\u3044\u305f\u3060\u304f\u3082\u306e\u300d\u306b\u8a18\u8f09\u304c\u3042\u308a\u307e\u3059\u3002 1M KPO4&nbsp;(pH &#8230; <\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[235],"tags":[],"_links":{"self":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/983"}],"collection":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/comments?post=983"}],"version-history":[{"count":5,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/983\/revisions"}],"predecessor-version":[{"id":2790,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/posts\/983\/revisions\/2790"}],"wp:attachment":[{"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/media?parent=983"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/categories?post=983"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.promega.co.jp\/resources\/faq\/wp-json\/wp\/v2\/tags?post=983"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}