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invitroバイオケミカルアッセイ試薬一覧

Assays for Biochemical Targets

This selector tool is designed to help you quickly find the right Promega assay for your protein target of interest. Find assays to measure the activity of a broad range of enzymes, from kinases and phosphatases to HDACs, hydroxylases and many more! Simply type in your enzyme of interest to find the correct assay.

Enzyme Name Gene Symbol Keywords Assay
Glycosyltransferase
Sugar chain synthetic enzyme, GalNAc
Polypeptide GalNAc Transferase 1 GALNT1 Glycosylation, neurological disease UDP-Glo Assay
Polypeptide GalNAc Transferase 4 GALNT4 Glycosylation, neurological disease UDP-Glo Assay
Sugar chain synthetic enzyme, GlcNAc
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase 3 MGAT3 Cell surface antigen UDP-Glo Assay
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase 5 MGAT5 Cell surface antigen UDP-Glo Assay
B 1,3-N-acetylgucosaminyltransferase 2 B3GNT2 Cell surface antigen UDP-Glo Assay
0-linked N-acetylglucosaminyltransferase OGT Glycosylation, neurological disease UDP-Glo Assay
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 POMGNT1 Glycosylation, neurological disease UDP-Glo Assay
Sugar chain synthetic enzyme, galactose
B-1,4 Galactosyltransferase 1 B4GalT1 Glycosylation, neurological disease UDP-Glo Assay
B-1,4-Galactosyltransferase 2 B4GalT2 Glycosylation, neurological disease UDP-Glo Assay
Sugar chain synthetic enzyme, sialic acid
ST3 Beta-Gal alpha -2,3-Sialyltransferase 1 ST3GAL1 Cell surface antigen, cancer CMP-Glo Assay
ST6 Gal Sialyltransferase 1 ST6GAL1 Cell surface antigen, cancer CMP-Glo Assay
Sugar chain synthetic enzyme, fucose
Fucosyltransferase 2 FUT2 Cell surface antigen, cancer GDP-Glo Assay
Fucosyltransferase 3 FUT3 Cell surface antigen, cancer GDP-Glo Assay
Fucosyltransferase 5 FUT5 Cell surface antigen, cancer GDP-Glo Assay
Fucosyltransferase 7 FUT7 Cell surface antigen, cancer GDP-Glo Assay
Fucosyltransferase 8 FUT8 Cell surface antigen, cancer GDP-Glo Assay
Sugar chain synthetic enzyme, mannose
Mannosyltransferase _ _ GDP-Glo Assay
Glycosylation enzyme
Blood Group B Transferase GTB Blood type, cell surface antigen UDP-Glo Assay
Bacteria pathogenic toxins
Clostridium difficile Toxin B Toxin B (TcdB) _ UDP-Glo Assay
Ubiquitin-proteasome
SUMO-adding enzyme
SUMO-activating enzyme subunit 1 / ubiquitin-like modifier activating enzyme 2 Heterodimer SAE1 / UBA2 Protein stabilization, transcription factor activity control AMP-Glo Assay
SUMO conjugating enzyme UbcH9 Protein stabilization, transcription factor activity control AMP-Glo Assay
NEDD additional enzyme
Ubiquitin-like modifier activating enzyme 3 UBA3 Protein stabilization, transcription factor activity control AMP-Glo Assay
NEDD8 activating enzyme E1 subunit 1 NAE1 (APPBP1) Protein stabilization, transcription factor activity control AMP-Glo Assay
Ubiquitin ligase
Ubiquitin Ligase E1 UBA1 Proteolysis / quality control AMP-Glo Assay
Ubiquitin Ligase E2 UBE2D2 / UbcH5b Proteolysis / quality control AMP-Glo Assay
Ubiquitin Ligase E3 CARP2 Proteolysis / quality control AMP-Glo Assay
Proteasome
Proteasome Chymotrypsin-Like, 
Trypsin-Like, Caspase-Like
PSMA / PSMB / PSMC / PSMD / PSME / PSMF / PSMG Protein degradation, protein quality control, ubiquitin disease, neurodegeneration, cancer Proteasome-Glo™, Cell-Based System
Proteasome Chymotrypsin-Like, 
Trypsin-Like, Caspase-Like
PSMA / PSMB / PSMC / PSMD / PSME / PSMF / PSMG Protein degradation, protein quality control, ubiquitin disease, neurodegeneration, cancer Proteasome-Glo™ Assay
Protein modification
Deadenylation
Poly A-Deadenylases PARN mRNA stability, gene expression control AMP-Glo Assay
Protein poly ADP- ribosylation
Poly ADP-Ribose transferases 1 PARP1 DNA damage repair, cancer AMP-Glo Assay
chaperone
Heat Shock proteins HSPs Protein synthesis, quality control ADP-Glo Assay
Epigenetics
Methylase
DNA Methyltransferase DNMT1, 3a Epigenetics, cancer MTase-Glo™ Assay
Histone Methyltransferase EZH1, 2 Epigenetics, cancer MTase-Glo™ Assay
EHMT1, 2 _ Epigenetics, cancer MTase-Glo™ Assay
SET7, 9 _ Epigenetics, cancer MTase-Glo™ Assay
PRMT5 _ Epigenetics, cancer MTase-Glo™ Assay
DOT1L _ Epigenetics, cancer MTase-Glo™ Assay
Histone demethylating enzyme
JumonjiC histone demethylases JMJD2A Cancer, glycolipid metabolism and metabolism-related gene expression Succinate-Glo assay
JumonjiC histone demethylases JMJD2C Succinate-Glo assay
JumonjiC histone demethylases JARID1A Succinate-Glo assay
JumonjiC histone demethylases UTX Succinate-Glo assay
Histone deacetylase
HDAC1-11 HDAC1-11 Epigenetics, cancer, gene expression control HDAC-Glo™ I / II Assay
HDAC4, 5, 7, 9 HDAC4, 5, 7, 9 Epigenetics, cancer, gene expression control HDAC-Glo™ Class IIa Assay
HDAC2 HDAC2 Epigenetics, cancer, gene expression control HDAC-Glo™ 2 Assay
Sirtuin deacetylase
SIRT1-6 SIRT1-6 Anti-aging, energy metabolism, SIRT-Glo™ Assay
Nucleic acid synthesis and degradation enzyme
Nucleotidase, AMP-degrading enzyme
5′-nucleotidase (5′-NT) or Ecto-5′-nucleotidase CD73 (NT5E) Liver function markers, gastrointestinal disease AMP-Glo Assay
Ligase
DNA Ligases _ Antibiotics, antibacterial agents AMP-Glo Assay
Nuclease
Ribonucleases RNase _ AMP-Glo Assay
Helicase
Helicases _ Transcriptional regulation, DNA synthesis ADP-Glo Assay
Signaling
cAMP-degrading enzyme
Phosphodiesterase 3B PDE3B cAMP signaling AMP-Glo Assay
Phosphodiesterase 4B PDE4B cAMP signaling AMP-Glo Assay
Phosphodiesterase 8A PDE8A cAMP signaling AMP-Glo Assay
Phosphodiesterase 10A PDE10A cAMP signaling AMP-Glo Assay
Phosphodiesterase 11A PDE11A cAMP signaling AMP-Glo Assay
Phosphodiesterase 3B PDE3B cAMP signaling cAMP-Glo Assay
Phosphodiesterase 4B PDE4B cAMP signaling cAMP-Glo Assay
Phosphodiesterase 8A PDE8A cAMP signaling cAMP-Glo Assay
Phosphodiesterase 10A PDE10A cAMP signaling cAMP-Glo Assay
Phosphodiesterase 11A PDE11A cAMP signaling cAMP-Glo Assay
Phosphodiesterase 3B PDE3B cAMP signaling PDE-Glo™ Phosphodiesterase Assay
Phosphodiesterase 4B PDE4B cAMP signaling PDE-Glo™ Phosphodiesterase Assay
Phosphodiesterase 8A PDE8A cAMP signaling PDE-Glo™ Phosphodiesterase Assay
Phosphodiesterase 10A PDE10A cAMP signaling PDE-Glo™ Phosphodiesterase Assay
Phosphodiesterase 11A PDE11A cAMP signaling PDE-Glo™ Phosphodiesterase Assay
Intracellular cAMP level change
Non-enzymatic assay (cAMP measurement) GPCR signal analysis GloSensor™ cAMP Assay
cGMP-degrading enzyme
Phosphodiesterase 5 PDE5 cGMP signaling PDE-Glo™ Phosphodiesterase Assay
Phosphodiesterase 6 PDE6 cGMP signaling PDE-Glo™ Phosphodiesterase Assay
Phosphodiesterase 9 PDE9 cGMP signaling PDE-Glo™ Phosphodiesterase Assay
Intracellular cGMP level change
Non-enzymatic assay (cGMP measurement) GPCR signal analysis GloSensor™ cGMP Assay
Low molecular weight GTP-ase
Ras Ras Cancer GTPase-Glo Assay
RhoA RhoA Cancer, cardiovascular disease, cell shape change GTPase-Glo Assay
RAB Rab5A Neurological disorders, immune disorders, membrane traffic GTPase-Glo Assay
Trimeric G protein
Gi alpha subunit GNAI1 cAMP signaling GTPase-Glo Assay
GTase activating protein (GAP)
Neurofibromin 1 NF1 Cancer GTPase-Glo Assay
Guanine nucleotide exchange factor (GEF)
Regulator of chromosome condensation 1 RCC1 Cancer GTPase-Glo Assay
GTase activating protein (GAP)
RanGAP1 RANGAP1 Cancer GTPase-Glo Assay
Kinase (proteins, peptides, lipids, sugars, etc.)
Protein Kinases (All) PKA, PKC, EGFR, FGFR and others Signaling ADP-Glo Assay
Metabolism and biosynthesis
Succinic acid Co synthesis, aerobic metabolism
Succinate CoA Synthetase SUCLG TCA cycle, aerobic metabolism Succinate-Glo assay / GTPase-Glo
tRNA synthetase
Aminoacyl tRNA synthetases _ Protein synthesis, metabolism AMP-Glo Assay
Energy metabolism enzyme / redox enzyme / other metabolic enzyme
Oxidase, Reductase, metabolic enzyme H6PD, GLUC1, 2, LDH, G6PD, Cancer, metabolism, diabetes NAD (P) H-Glo™ Detection System
Hydrogen peroxide detection
Catalase, Ascorbate peroxidases, NAD (P) H oxidase, Peroxidase CAT, APX, NOX, GPX Cancer, energy metabolism, ROS-Glo™ H2O2 Assay (H2O2 detection only)
Cytotoxicity (apoptosis)
caspase 3/7 CASP3, 7 Apoptosis Caspase-Glo® 3/7 Assay
caspase 8 CASP8 Apoptosis Caspase-Glo® 8 Assay
caspase 9 CASP9 Apoptosis Caspase-Glo® 9 Assay
caspase 1 CASP1 Apoptosis, inflammation, inflammasome Caspase-Glo® 1 Assay
ADME
Drug metabolism
CYP3A4 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP3A4 Assay with Luciferin-IPA
CYP3A4, CYP3A5, CYP3A7 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP3A4 Assay (Luciferin-PPXE) DMSO-Tolerant Assay
CYP3A4, CYP3A5, CYP3A7 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP3A4 Assay (Luciferin-PFBE)
CYP1A1, CYP1B1, CYP3A7 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP1A1 Assay (Luciferin-CEE)
P450-Glo™ CYP1B1 Assay (Luciferin-CEE)
CYP1A2, CYP2C8, CYP2C9, CYP2J2, CYP4A11, CYP4F3B, CYP19 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP1A2 Assay (Luciferin-ME)
P450-Glo™ CYP2C8 Assay (Luciferin-ME)
CYP1A2 _ Drug metabolism P450-Glo™ CYP1A2 Induction / Inhibition Assay
CYP2B6 _ _ P450-Glo™ CYP2B6 Assay
CYP2C9 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP2C9 Assay (Luciferin-H)
CYP3A4, CYP3A5, CYP3A7, CYP4F12 _ Drug metabolism, liver cell differentiation P450-Glo™ CYP3A4 Assay (Luciferin-BE)
P450-Glo™ CYP3A7 Assay (Luciferin-BE)
CYP2C19, CYP1A1, CYP1A2 _ Drug metabolism P450-Glo™ CYP2C19 Assay
CYP2D6, CYP1A1, CYP1A2, CYP2B6 _ Drug metabolism P450-Glo™ CYP2D6 Assay
CYP3A7 _ Drug metabolism Luciferin-3A7
CYP4A11 _ Drug metabolism Luciferin-4A
CYP4F2, CYP4F3 _ Drug metabolism Luciferin-4F2 / 3
CYP4F12 _ Drug metabolism Luciferin-4F12
CYP2J2, CYP4F12 _ Drug metabolism Luciferin-2J2 / 4F12 (ester)
High activity: CYP1A1, CYP1A2, CYP1B1, CYP2D6 
moderate: CYP2B6, CYP2C9, CYP2C19, CYP2J2, CYP3A4, CYP3A5, CYP4A11, CYP4F2, CYP4F3A, CYP4F3B, CYP4F12, CYP19
_ Drug metabolism Luciferin-MultiCYP (ester)
Drug transporter
P-glycoprotein 1 Pgp1 / MDR1 P-glycoprotein 1 Pgp-Glo™ Assay System with P-glycoprotein
ABC transporters Pgp1 / MDR1, MDR2, MDR3 Drug metabolism ADP-Glo Assay
Monoamine oxidase
Monoamine oxidase MAOA, MAOB Drug metabolism, Phase I enzyme MAO-Glo™ Assay System (by cell species)
N-acetylated
NAT2 NAT2 Drug metabolism, Phase II enzymes Luciferin-NAT2